Coding

Part:BBa_K1973037:Design

Designed by: Alvaro Escobar Doncel   Group: iGEM16_UPO-Sevilla   (2016-10-11)


nasR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 871
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 484
    Illegal NgoMIV site found at 750
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 880
    Illegal SapI.rc site found at 1030


Design Notes

We have included the sequence of RBS BBa_B0034 and the scar it should have been in case of the clonning of this part. When we disigned the sequence to synthesize, we change the nucleotide in position 159 in order to remove a PstI restriction site.

Source

It was obtained by synthesis.

References